Prevalence of Multidrug Resistant Bacteria Isolated from Infected Wound Patients in Tertiary Care Hospital of Rajshahi

Background: The threat of Multidrug resistant bacteria on the overall health sector has led to its recognition as the deadliest bacteria in the world. Most of the multidrug resistant bacteria are resistant to commonly used antibiotics including 3rd generation of cephalosporin, Fluroquinolones and carbapenems also. So, treatment of different infections caused by multidrug resistant bacteria are gradually becoming difficult and it also increased the mortality and morbidity. Objective: The aim of the study was to detect multidrug resistant bacteria isolated from infected wound patients in Rajshahi region. Materials and Methods: A cross sectional type of descriptive study was done during the period of July 2017 to June 2018. Wound swab was collected in different surgical units of Rajshahi Medical College Hospital. The specimens were inoculated in blood agar, nutrient agar and MacConkey’s agar media and incubated aerobically at 370 C for 24 hours. Susceptibility tests of the bacterial isolates were done by using the modified Kirby Bauer disk diffusion method on Mueller Hinton agar media. Multidrug resistant bacteria were identified by disk diffusion method against different classes of antimicrobials. Results: Out of total 250 samples, Culture yielded growth were 213(85.2%) and total 231 bacteria were identified. Among them 136 (58.8%) isolates were gram negative and 95 (41.2%) isolates were gram positive. S. aureus was the predominant organism 71(30.8%) followed by E.coli 48(20.8%), Aeruginosa 47(20.3%) and Klebsiella spp.20 (8.7%).Overall, 231 bacterial isolates were obtained and 135 (58.4%) were identified as MDR. The overall MDR among gram positive and gram-negative bacterial isolates were 55.8% and 60.3% respectively. Among gram positive bacteria’s. aureus, Cons, and Enterococcus spp.39(55%),08(57.1%) and 06(60%) were identified as MDR respectively. Among gram negative bacteria, E.coli, Aeruginosa, Klebsiella spp., Proteus spp. and Acinetobacter spp. 28(58.3%), 30(63.8%), 12(60%), 08(57.1%) and 04(57.1%) were identified as MDR respectively. Vancomycin, linezolid and Imipenem were the most sensitive drugs against gram positive bacteria. Colistin and Imipenem were the most sensitive drugs against gram negative bacteria. Conclusion: The study highlights a high rate of multidrug-resistant bacterial pathogens causing wound infections, emphasizing the urgent need for effective antimicrobial stewardship and infection control measures.